Application of Dried Blood Spots and Serum Samples for the Determination of Vitamin D Metabolites in the Group of Healthy Women and with Hashimoto’s Thyroiditis

Abstract Purpose The relationship between Hashimoto's thyroiditis and vitamin D concentration was already presented in many studies. aim of this study to analyze the differences metabolites healthy women with (HT). Methods quantitative analysis five carried out using liquid chromatography coupled tandem mass spectrometry. analyzed materials were serum dried blood spots (DBS). results obtained for two also compared. Results No statistically significant found mean 25(OH)D 3 , 2 24,25(OH) 1,25(OH) test control groups. However, a strong correlation metabolites. Conclusion showed that disease had similar Research proved DBS is good alternative serum. not (17.0 15.5 ng mL ?1 DBS, respectively). can be successfully used research on large group people, since process material collection, as well sample preparation, fast simple. It easy transport store, requires small volume blood.